37 Blog Post Ideas for New Freelance Writers

Coming up with blog post ideas can be hard when its your job! Freelance writers often have to come up with blog topics and pitch their ideas to their clients or editors. And to top it all off, if you have a blog or personal blog – which every freelance writer should – having fresh ideas can take a toll. Ive been freelance writing for a little over a year now and I have over 20+ clients, two blogs I write and manage and two other blogs where I guest contribute. So, you can say there are days when Im totally unable to come up with a blog post idea and Im scrambling. Does that happen to you? You land several clients in your niche and now you have to come up with three topic ideas in the same niche! While the best clients are the ones that give you free range to pitch ideas and come up with your own topics – for me – these clients can be more time consuming. So, to make sure Im working smarter and not harder, Ive thought of 37 blog post ideas for your clients or for your blog. Bookmark this post and refer back to it for more inspiration! 1. Use Title Generators I dont know about you, but if I read headlines in my niche, this is enough to get the creative juices flowing and help me come up with several topics very easily. The best   title generator that gives you oodles and oodles of ideas is the Tweak Your Biz Title Generator. Just enter your topic, let it know if its a noun or verb and hit submit. Heres my result for email marketing. You can write great blog posts just by look through these headlines: 10 Effective Ways to Get More Out of Your Email Marketing Email Marketing Doesnt Have to Be Hard – Read These 10 Tips 10 Creative Ways You Can Improve Your Email Marketing Tweak Your Biz isnt the only headline generator. Others include Portent and SEOPressor. 2. Look At Your Archive If youre struggling coming up with blog post ideas for your blog, go through your archive. See what you have already written. This can give you ideas about any holes or gaps you might have missed. For example, I know for this blog, I havent done a post on tools for freelance writers. Ive realized that a lot of new freelance writers arent aware of what tools to use for your business. This is something I could talk about on this blog. For your clients, if their blog has many posts, go through them and see if you can add a new perspective to older content. For me, since I guest post on FreelancerFAQs – a site I manage and own – I often look at old posts on this blog and use that to help me write a   post on FAQs. For example, I wrote 6 Foolproof Marketing Tips to Help You Become a Better Freelance Writer on this blog. For FreelancerFAQs, I ended up writing a post called, How Do I Promote Myself as a Freelance Writer? While both posts talk about marketing yourself, each one is unique and provides lots of different tips. 3. Look at Competitors Blogs Dont think no one does this! The best bloggers and freelance writers always grabs an idea or two from competitors in their niche or clients niche. For example, with this blog, since Im writing about freelance writing mostly, Ill look to The Write Life, FreelancerFAQs, Be a Freelance Blogger and even hit up personal freelance writers blogs like  Lauren Tharp for ideas. I remember looking at this title on The Write Life and thinking I can do a post on that subject.For one of my ghostwriting clients, I often have to look at other blogs in their niche to draft up some topics for the month. It doesnt take long, but its a lot easier to do this than to come up with completely original ideas, because if you already dont know, most blog post ideas have been recycled. 4. Read Posts in Your Niche Part of being a freelance writer is knowing the top trends going on in your niche. This helps you learn any new products, tools or applications that your client might like you reporting on their blog. Since Im in the digital marketing niche I often try to read a post or two from the following blogs: Neil Patel QuickSprout Twelveskip Smart Blogger Hubspot Unbounce Kissmetrics I dont do it all the time, but when I have some time Ill check out these sites and go through their blog and find some good reading material. 5. Take a New Angle on a Known Topic As I mentioned before, most everyone has written about all the topics in your niche. Its hard to find something no one has heard of. What you can do instead, is provide a new angle on a known topic or something thats been written about before from many people. For example, for the freelance writing niche, I know theres been talk about displaying your rates on your site. Most say that its better to display them since you wont get any low-ball offers and youll end up attracting your ideal client. So, why not flip that around and write a post on   Not Posting Your Rates Makes You More Money. Its a new angle on a known topic. 6. Start a New Blog! Wh-what? Why would starting a new blog give you more ideas? A new blog MEANS you NEED more ideas. Well I disagree. I dont know about you, but Im a creative person. Thats why I love doing images for my blogs and clients and thats why I am a freelance writer. I recently started a new blog called TwinsMommy. Its not a mommy blogging site where all I talk about are my twins. Its actually a work from home site for moms that want to go from mom to mompreneur. Its in a similar niche as freelance writing, but its much broader. And you know what? I have about twenty blog post ideas for that blog already. And, because of all that creative juice flowing, I have several ideas for this blog too! So, why not start a new blog? 7. Turn an Infographic Into a Blog Post Go to Pinterest and search for infographics. Heres one on branding. Use this to inspire you and write your own branding post to help freelance writers, entrepreneurs, small businesses etc.. 8. Turn a Podcast Into a Blog Post Just like you can use inforgraphics to help you come up with blog post ideas, you can also use podcasts. Little Zotz has done podcasts on freelance writing. Listen to some of those and use what youre learning from those podcasts to help you come up with a blog post idea for your blog. 9. Turn a Video Into a Blog Post Finally, check out some YouTube videos of bloggers in your niche. You can get loads of great blog post ideas for your blog and your clients blog. Freelancer Lizzie Davey has a YouTube channel for her Wanderful World blog. Her videos talk about freelancing in general. Looking to other freelancers YouTube channels or bloggers/marketers in your niche can help you generate tons of blog post ideas. 10. Do a Case Study People love learning from others. If youre successful at what you are doing, plenty of people want in on how you were able to achieve this. What better way to explain your process then with a case study on your blog or even your clients blog? Over on one of my clients blog, I wrote a post about my email marketing sales funnel. I pitched them my idea and they said go ahead. Over on my TwinsMommy blog Im doing a case study on growing that blog. Im going to do monthly reports on generating more traffic, readers and money from TwinsMommy. Thats a post every month, which leaves me with one less idea to come up for my monthly content schedule. 11. Use Entertainment for Blog Post Ideas Its important as freelance writers to do more than just write! Watch a movie, follow a TV show, read a book or listen to the news. By doing this you can draw from those types of entertainment and relate it to your industry. Course student Kate and shared on her blog how The Walking Dead relates to Freelance Writing.Want another example? Heres Francesca Nicasios post on her blog, Be a Freelance Writer, talking about how the show How I Met Your Mother can teach you about finding clients. Do you have a favorite TV show, book, sports team, movie or actress you can use to create a blog post in your niche? 12. Use BuzzSumo or Quora For Ideas To use these tools, you first need a keyword or general idea of what you want to write about. So, for example, if one of your clients is in the app business, then finding topics around that particular app or apps in general will help you out. Over at BuzzSumo, I typed in best apps for parents and got this result: From the results there are several topic ideas I can use for my clients. Quora works in a similar way. Instead of typing in a keyword, you ask a question. If you need information on productivity tools Quora can help you. 13. Do a Project Wrap-Up Post Your readers are interested in what you do. So why not treat them with a post on where youre writing or where you are published? Doing an end of the month wrap-up post on where youve been published is a great way to inspire other writers and to let prospects know you are in demand. On this blog Ive written two posts on where Ive been writing. Freelance writer Sharon Hurley Hall does a Blogging Portfolio on her blog. And Katharine Paljug has done monthly wrap-ups over on her freelance blog. 14. Post a Question on Facebook Facebook is a great place to generate lots of blog post ideas. I belong to several entrepreneur FB groups, small business FB groups and freelance writing groups. The other day I posted this question and got some great responses that helped me see what new writers are struggling with. 15. Use Real Life for Your Blog Post There are many ways to use your own life as a muse for your blog. If you have children, pets, wacky family members, or you are a part of a gym or class, you can use that for inspiration. For example, I was able to score a paid gig from a free guest post. I thought that was a cool thing and that other new writers would benefit from my story. So, I made a post on how I was able to turn a guest post into a paid gig. 16. Broaden Your Niche Your niche is too focused! How many posts on blogging or freelance writing can you write about? Theres going to be a time when youve written about all there is in a niche. What do you do? Well, you broaden your niche. For me, this blog is purely about helping new freelance writers. But, again, theres only so much I can write about that relates to brand new freelance writers. But, you know what? I can also write about blogging in general (like this post here) or writing techniques ( like my editing process post) or freelancing in general. While its ideal to stick to ONE niche, its more than okay to broaden it and include related topics. This will help make your blog live longer! 17. Condense a Series Did you do a series for your client or on your blog? Ive done a couple series on this blog and whats great is that I can take a series, condense it and create a new post. Thats what I did with my Freelance Writing Jobs for Newbies series. I created a new post, The Complete Guide to Getting Started Freelance Writing From Scratch. While the information in my guide is different than my series, its based on that series since the topic is about getting started freelance writing. So, if you have a series on your blog, take a look at it and see if you can condense it to make one big post. 18. Do a List Post Listicles are easy to do and people seem to love them. My 20 Ways to Find Freelance Writing Jobs as a Beginner has over 45,000 shares! Clients like list posts because its highly shareable. So, dont be afraid to write list posts for your blog or your clients, especially if their end goal is to generate more traffic and leads. 19. Expand on a Post An easy way to come up with a blog topic is to find an old blog post and expand on it. Maybe now you know more about that topic or you have a new angle to share. I find that when I look back at old posts, I can see lots of areas where I can expand and create new content. For example, I wrote a post about the importance of having a writer website. Recently I had a coaching call with a writer and made some suggestions to optimize her website. As a result I wrote about it and included a case study, which just expanded on my previous post about having a writer website. Both these posts talk about your writer website, but I was able to make a new angle (attracting clients) and offered a case study on how to improve your writer website. 20. Recruit a Guest Writer Sometimes youre just too busy to write a post for your blog. You dont want to neglect your blog and, in fact, you should be treating your blog like a client. But, at the same time, you dont want to just put out a junky post. So, what do you do? Well why not ask a fellow freelance writer to write a guest post for your blog? You are still providing lots of value to your readers, and it just gives you more time to work on your client work. Recently I had one of my course participants write a guest post talking about a pitching challenge I held for my students. It was a great post on how it changed her whole freelance writing career and proved to be highly valuable for new writers. If youre short on time, enlist a blogger friend and see if they wouldnt mind contributing to your blog. 21. Write a Sequel to a Post Having themes on your blog is a good thing. If every month you follow up on the same theme, you can build a following. Maybe theres an old post youd like to re-hash. Make a sequel to it and provide even more tips and better information. Its great to recycle old blog posts and make them even better than before. 22. Take a Subheading and Turn that Into a Post Its always fun to look through old posts and see if you can turn a subheading into a blog post. Flushing out an idea is a challenge, but for freelance writers, its something that is required. Good writers can expand on topics and condense pages of books. So why not practice this skill with your blog posts? Awhile back I wrote a post on Discover These 4 Freelance Writing Mistakes That Are Costing You Clients and Cash. One subheading in that post is, Youre Not Charging Enough. I was able to flush out that idea and create a post on what to do before raising your rates. 23. Do a Behind the Scenes Post People like to learn about you. They want to see whats happening in your business. Why not let them? Ive written a post on my freelance writing business as have other bloggers like Nathan Barry or Adam Connell. Update your readers on whats going on in your business. It inspires them and helps them grow their own business. 24. Write a Goals Post This is along the same lines of an update post. Create some yearly or monthly goals and share them with your readers. Theyll appreciate it and it will help deepen the connection with them. This is what Tom Ewer of Leaving Work Behind did back in 2015. 25. Reflect on The Past Its always fun to think about how you succeeded and what you learned. For me, as a new freelance writer I learned a lot in my first year. I thought my readers would benefit on how much I grew as a writer and since I enjoy reading about other freelance writers, my readers would too. For example, Karen Marston of Untamed Writing wrote a big post on all the phases of her blog. It gives the readers a glimpse of her brand and business growth. 26. Host a Giveaway or Contest A great way to increase your email list and readership is to host some sort of giveaway or prize. Of course anything you give away will come at your expense (unless you have connections), but its a small price to pay if you want to grow your blog. Sophie Lizard over at Be a Freelance Blogger regularly holds PitchFest, which is a content for freelance writers. If you win, you earn $100! Over on FreelancerFAQs we held a giveaway as a way to generate buzz around the new site. If youre stuck on a blog post, you can always hold a contest! 27. Run a Survey Then Post on Your Blog A great way to get some blog post ideas is to just ask your readers. It can tell you a lot about what their struggles are or what their aching to find out. Writer Bryan Collins of Become a Writer Today did a survey with his readers and posted the results. Its easy to create a survey. You can use SurveyMonkey or Google Forms. 28. Review a Product or Tool Sometimes the most useful blog post is a review of a product or tool. If you find that you are familiar with Skitch or Canva or Tailwind, why not write a post about it? Maybe you took a course and want to write about it or compare it to other courses. This is what the Lazy Millennial did. They wrote a review post on my freelance writing course. 29. Write a Comprehensive Guide People love guides. If you write the ultimate guide, the complete guide or the advanced guide, your readers or your clients readers will love it and learn a lot from it. Neil Patel is known for his comprehensive guides over on QuickSprout. Why not make a complete guide or series of guides for your blog or your client? Ive written complete guides for a few of my clients as they make great content for their blog. Ashlee Anderson from Work From Home Happiness has a section for guides on her blog. 30. Do a Round-Up Post People love round-up posts. They love contributing to it and people love reading and sharing it. Tor Refsland is known for his epic round-up posts. One of his round-up has 117 contributors! Thats freaking a lot. Dont worry if you dont know even 50 people in your niche. Ive done two round up posts with less than 20 people. 31. Feature Other Blogs Your blog doesnt have to be all about you! Share the love and feature other bloggers and blogs. One popular post I did was 10 Best Sites to Help You Become a Successful Freelance Writer. 32. Do a Spotlight Series A popular way to have a full content schedule is to do a spotlight series. This means you pick one person to highlight on your blog. Lauren Tharp does a spotlight series called, People I Know and even featured me! Lorraine Reguly has, in the past, done a F.I.T.S. (Freelancer in the Spotlight) Series featuring freelancers. And I did a Confessions of a Freelance Writer series on my blog and interviewed several freelance writers I look up to. 33. Do a Seasonal Post For me, I didnt do a seasonal post for the longest time. I felt that evergreen content (content that isnt contingent on a time frame) was the best sort of content you can provide on your blog. But, lo an behold, I was running out of blog post ideas and I wanted to spend time with my family. So, I bit the bullet and wrote some seasonal posts. 5 Year End Tasks Freelance Writers Must Do My 2015 Freelance Writing Goals – Success or Fail? 5 Ways Freelance Writers Can Motivate Themselves After the Holidays And you know what? Im happy that I did. I plan to do that again next holiday. 34. Write a Post On an Obscure or Unknown Fact Maybe you found a shortcut to some complex task or found an easy way to do a typical task. I recently created a post on 2 Obscure Ways Freelance Writers Can Create Samples. I had learned some cool ways brand new freelance writers could score some clips and I wanted to share them with my readers. This idea can also be adapted for your clients. If you work with a B2B company and found some neat hack with their product, let them know and pitch that idea for their blog. 35. Write a Mistakes Post People love to read about the worst things or the top mistakes of something. Normally, people dont want to fail, so posts like these are enticing for most people. Ive done my share of mistake-type posts on this blog! 36. Write a Why You Got Started Post Its fascinating to read how successful people first got started. How did they get started and why did the choose to go that way? You can pitch this idea to your clients if you know their brand inside and out and you can do this type of post on your blog. 37. Do a Series Instead of trying to come up with a months worth of blog topics, why not think of one topic and write several posts on it? For example, Sarah Titus on her blog is doing a 52 week series on how to build your credit score. Ive Got Your Blog and Clients Blog Covered Ha, what I mean is, I covered 37 blog post ideas for you and your clients. If you write one blog post a week, thats 9 months of content Ive suggested! Do you have some more blog post ideas I didnt mention? Please share them with me! Dont forget to grab your free schedule for your blog posts. Oh, and please Pin me!

Fairest by Gail Carson Levine Notes Essays

Fairest by Gail Carson Levine Notes Essays Fairest by Gail Carson Levine Notes Paper Fairest by Gail Carson Levine Notes Paper In front of my face when guests arrived, foolish practice, because It raised curiosity and concealed little. This part reminded me of myself because I have stupid habits. Also, I felt sad that AZ would think that she would have to hide herself or feel ashamed about what she looked like. 87 She was our ruler now! Everyone in the room and those outside knelt and swore their loyalty. I knelt, too, and she swayed against my shoulder. How could she rule? She didnt know Oratory. How frightened and grief stricken Id be in her place. This makes me feel angry but sorrow because Vii wasnt fit to be the queen, she didnt know how to rule so it wasnt fair that the country was put In such bad hands. But, I feel bad for her because she was scared to have to rule the kingdom. Also, she didnt deserve the king because she didnt love him; she Just wanted him to love her. 9-100 In Amount, at the Featherbed Inn, Where I once lived, my mother rakes up the fire. My father wakes the cook, who cannot cook today. Cream curdles; milk sours; eggs break; onions rot. My father and my mother put down their forks. In his castle, the king swallows enough but air. His life has narrowed, but his thread winds on. Should the king come to Amount, eyes wide, legs hale, mouth full of words . .. Cakes would bake themselves, mares s hoe themselves, roads pave themselves. My mother would don her damask gown. And I would sing until the sun cheered and the inn dissolved in music. : This song shows me that AZ really cares about the king and hopes he gets teeter and about Amount, the Featherbed Inn, and her family because when she put them into her song not only did it express her love, but also her sorrow. Thought this makes me wonder if everyones song were somewhat like this or if Seas was one of a kind and how Queen lavas song went and the reactions of the people at the sing. 316 At the sing I sat next to Koori, with Coho slating at attention between us. King Oscar and VII entered. The king seated himself next to me and placed VII on his other side. I tightened my grip on Coirs hand. This shows me that the king had forgiven AZ and anted to show her that and that he would keep Vii under control. Also, when AZ grabbed Coirs hand that she knew he would protect her and she would be safe with him and she loved Koori. Title: Fairest Author: Gail Carson Levine I developed the habit of holding my hand in front of my face when guests arrived, foolish practice, because it raised curiosity and concealed little. This part reminded know how to rule so it wasnt fair that the country was put in such bad hands. But, I feel bad for her because she was scared to have to rule the kingdom. Also, she didnt serve the king because she didnt love him; she Just wanted him to love her. 99-100 king come to Amount, eyes wide, legs hale, mouth full of words Cakes would bake kind and how Queen Aviss song went and the reactions of the people at the sing. 316 At the sing I sat next to Koori, with Coho sitting at attention between us. King Oscar and Vii entered. The king seated himself next to me and placed Vii on his other side. I tightened my grip on Coirs hand. This shows me that the king had forgiven AZ and grabbed Coirs hand that she knew he would protect her and she would be safe with him and she loved Koori.

Their Eyes Were Watching God Essay Example | Topics and Well Written Essays - 500 words - 5

Their Eyes Were Watching God - Essay Example The plot of this novel is set in a postwar era when it was difficult to accumulate wealth, but Logan still managed to offer physical security and shelter. This paper, therefore, analyses the difference between Nanny and Janie’s worldviews in relation to the theme of Love and Relationship versus independence as presented in chapter three scene. Janie perceives Nanny as a former slave who had no independence and, therefore, she would have such a perception. Her life has been characterized by hardship and poverty, and any progress to acquire material wealth has been derailed by her skin color. Therefore, according to her perception, Logan who owns his own land and has financial independency is the ideal husband she could dream of when she was still young. However, according Janie, a woman in her current age should pursue something more than material wealth from a man. She is looking for some kind of completion that offers both emotional connection and passion. Emotional and physical connections are significant in her life and inseparable from her understanding of love. This is the reason she describes Logan as â€Å"ugly† and â€Å"he doesn’t speak beautifully to her† (Hurston 27) when explain why she does not love Logan. This implies that she neither feels physical, nor intellectual, nor emotional conne ction to him. In the pursuit for physical and emotional connections to a man, she decides to run away with Jody. She believed that her real man was hiding somewhere. Janie believes â€Å"God tore down the old world every evening and build a new one by sun-up. It was wonderful to see it take the form with the sun and emerge from the gray dust of its making† (Hurston 32). This is a sign of Janie’s romantic desires. She is even ready to compromise or abandon these desires for the possibility of change. In conclusion, the conversation between Nanny and Janie, in Chapter

Financial Management Essay Example | Topics and Well Written Essays - 1500 words - 4

Financial Management - Essay Example The deal was not an easy one for Google considering that the company had to put in a lot of effort for obtaining the regulatory approvals for the same (Reuters, 2012). The deal was announced at $40 per share which added up to a total of $12.5 Billion which was at a premium of 63% to the price of Motorola shares at the end of August 12, 2011. The acquisition of Motorola was aimed at protecting the viability of Google android considering the fact that Google was recently facing a threat due to patent war existing throughout the industry, due to which the major android manufacturers like HTC and Samsung were being sued by giants like Microsoft and Apple for the infringement of patents (Gaughan, 2011, p.5). Google announced that it will run Motorola as an independent business. The deal received approval from the shareholders as well as from the United States Department of Justice and the EU in early 2012. The approval from Chinese authorities followed and the deal was completed on May 22 , 2012. The deal represented Google Inc.’s biggest challenge to Apple Inc., which was the leading player in the market of mobile phones and tablets. Economies of scale A vertical merger generally has a lower potential for economies of scale than a horizontal merger but the merger of Google and Motorola saw the achievement of economies of scale in both financial and risk bearing economies (The Economist, 2008). Technical, organizational, bulks buying as well as financial economies of scale were achieved from the merger (Thompson, 2012). Coordination improved in terms of cost fit, timing fit, size and communication fit within the business (Arnold, 2005, p.45). The merger immediately showed a success effect by increasing the market share in the world market for smartphones from 46.9% in the first quarter of 2012 to 68.1% in the last quarter of 2012. Economies of scale was achieved by getting other byproduct benefits such as the development of the next generation device for mobil e computing, extra services, for example, advertising to living rooms through Motorola'scable TV boxes which helped in boosting the staggering set top box business, in smartphone designs aimed to fulfil the government regulations and competing with Microsoft’s new release of Windows phones. The company achieved a higher output with lowering the average cost, thus increasing the profitability and ensuring lower price for the customers (Rosenbaum, 2012). Economies of vertical integration The move to buy Motorola had a positive impact on the margins. Major phone manufacturers show a trend of having slim margins (Neale, 2004). But market leaders like Apple and Samsung have been known to maintain a margin of 40-50%. Google maintained net profit margin of 25% without having to subsidize the cost of manufacture in order to attract new clients. The merger showed a 100 to 150 basis points positive impact on the profitability. The merger enabled Google Inc. to supercharge the Android e cosystem and enhanced it competitive features in the mobile computing sector. The merger also opened up new opportunities for cross licencing. Combining complementary resources One of the main motives behind the merger was Google’s intention to accelerate innovation by combining the technical resources of Motorola Mobility. Another primary reason was that Google wanted to acquire the huge number of patents that Motorola had. The acquisition of these patents was likely to give the company a high competitive strength against its

Physicological Characterization of M.VITRJS5

Physicological Characterization of M.VITRJS5 Monica Samal, Teena Patra, Suthindhiran K Abstract Magnetospirillium sp. is a Gram – negative ÃŽ ±-proteobacterium which has the ability to move towards the geomagnetic field lines of Earth’s magnetic field. The group of bacteria is collectively called Magnetotactic bacteria (MTB). MTB produce intracellular organelles called magnetosomes which help them in its search for optimal living conditions in complex environment. MTB is a gradient – requiring microorganism that are difficult to replicate in growth medium in the laboratory. In this work the physiological characterization of MTB strain Magnetospirillium sp. VITRJS5 was done as well as a modified growth medium for the growth of Magnetospirillium sp. VITRJS5 was developed. The media components are studied based on the physiological characteristics of the bacteria. The role of electron donors, electron acceptors, nitrogen sources, carbon sources, iron containing compounds, phosphates, reducing agents, on the growth of the bacteria in the MS1 media was studied. The growth of the bacteria in the novel modified MS media was analysed and compared with commercial MTB media such as MSGM (Magnetospirillum growth media) and Schuler’s oxygen – sulphur gradient media. An increase in growth is observed in the modified MS media compared to the commercially available media. 1. INTRODUCTION: Magnetotactic bacteria are fastidious microorganisms that represent a morphologically and physiologically diverse group of bacteria. The presence of intracellular, membrane bound nano-sized magnetic particles called magnetosomes assists the bacteria in sustaining an optimal position in redox gradient habitats. Magnetosomes are crystals of iron mineral which consist of either iron oxide (Fe3O4) or iron sulphide (Fe3S4). A combination of aerotaxis, magnetotaxis and chemotaxis helps MTB to move away from oxygen in surface water, thus directing them in maintaining an optimal position in and around the oxic-anoxic interface (OAI). Magnetospirillum sp. is the most studied and well understood group of bacteria among MTB. Magnetospirillum sp. was mostly isolated from fresh water habitats and many pure cultures were available in many laboratories around the world. Magnetotactic Bacteria (MTB) species have characteristic organelles called magnetosomes that contain magnetic crystals. These magnetosomes give them the ability to move in response to the earth’s magnetic field (magnetotaxis). MTB were first observed by Salvatore Bellini in the year 1963 and collected from different freshwater environments near Pavia, Italy [1,2]. The most studied species of Magnetospirillum genus are species of Ms. gryphiswaldense [5], Ms. Magneticum[7], Ms. magnetotacticum [8]. The MTB strains isolated from marine or brackish environments include: the coccoid strains: MO-1[9] , Magnetococcus marinus strain MC-1[10] and Magnetofaba australis (IT-1)[11] ; the vibrioid strain: Magnetovibrio blakemorei strain MV-1[12] and the spirilla Magnetospira thiophila (MMS-1)[13] and Magnetospira sp. strain QH-2[14] ; all of which belong to the Alphaproteobacteria. Most magnetotactic bacteria (MTB) are known to be phylogenetically similar to the Alphaproteobacteria, Gammap roteobacteria and Deltaproteobacteria classes of the Proteobacteria and the Nitrospirae phylum with response to magnetosome formation[6]. Magnetotactic bacteria are known to thrive in sediments or chemically stratified water columns, where they occur mostly at the oxic-anoxic interface (OAI), the anoxic regions of the habitat, or both [3]. Although the detection of these in samples is relatively simple [4], magnetotactic bacteria are a fastidious group of prokaryotes, and special culture conditions are necessary for their isolation and cultivation. There is clear evidence that the availability and diversity of MTB in different environments is majorly influenced by salinity and also, iron availability, temperature and concentrations of sulfur and nitrogen compounds are also seen to be clearly important in the distribution of MTB. The respiratory forms of metabolism of genus Magnetospirillum are either chemoorganoheterotropic, using organic acids and carbon source or electron donors. Mostly Magnetospirillum sp. capable of autotrophic and mixotrophic growth and utilize nitrate as alternative terminal oxygen acceptor. Magn etospirillum are obligate microaerophile that requires oxygen even when nitrate is present in the medium. The present study,examined the phylogenetic affiliations and physiological characteristics of Magnetospirillium sp. VITRJS5. To characterize physiological parameters, microaerophillic batch experiments were performed to determine the following: (1) growth pH and temperature ranges, (2) Optimum electronic donor/carbon source and electron acceptor. (3) optimum nitrogen and phosphate source (4) optimum Iron source (5) optimum reducing agent/ sulphur source. Once the preliminary screening of the compounds over different concentrations of the nutritional requirements is determined to develop a modified growth media. 2. Methods and materials: 2.1. Media and microorganism Magnetospirillum VITRJS5 (Accession number: KM289194) is a novel MTB isolated from the fresh water sediment. They are spiral in shape and move towards the earth’s magnetic field. They have polar flagellum at each end of the cell. The strain was obtained from Marine biotechnology and biomedicine lab, VIT University. The bacteria were sub-cultured in Magnetospirillum growth media (MSGM) under microaerophillic conditions Magnetospirillum VITRJS5 was initially grown in previously standardized minimal media MS1 to analyse the growth conditions in the presence of electron donors and acceptors. MS1 media per litre containing K2HPO4 – 850mg, Na2HPO4 – 850mg, NH4H2PO4 – 500mg, MgSO4 – 100mg, FeSO4 7H2O – 5mg, Na2MoO4 – 0.2mg, EDTA – 10mg and Mineral solution 10ml Electron donor and acceptor has to be added accordingly. From the earlier studies, the optimum temperature and pH for the growth of Magnetospirillum is 28 °C and 7.5 pH. 2.2 Determination of electron acceptors and concentration of selected acceptor: For this, 50 ml of MS-1 media was added in five serum bottles and 50 mg of electron donor which in this case is sodium acetate (CH3COONa) was added to each bottle. Then 50 mg of five different acceptors were added in different bottles. These five acceptors are nitrite, selenite, nitrate, thiosulphate and sulphate. Nitrogen was sparged to the media to make it anaerobic, closed with butyl rubber stopper and sealed with aluminium caps. All the anaerobic culturing was performed according to Hungate 1950[15]. The Magnetospirillum VITRJS5 was inoculated to MS1 media. These bottles were kept for overnight incubation in a shaker incubator. Now the different concentrations of selected acceptor is determined .It is done for concentration of 5mM, 10 mM , 15mM, 20 mM and 30 mM . Steps for anaerobic culture was repeated. The absorbance is checked in UV-VIS spectrophotometer at wavelength of 595 nm. 2.3. Determination of electron donors and concentration of selected donor: For this, 50 ml of MS-1 media was added in five serum bottles and 50 mg of electron acceptor which in this case is nitrate was added to each bottle. Then different donors were added in different bottles. These donors are methanol (250  µl), ethanol( 50 µl), glycerol (50 µl), pyruvate (82.5 mg/ 15mM) , citrate (221 mg/ 15mM),succinate (202.6 mg/15mM), lactate(110 µl/15mM), glucose (135.12 mg/15mM), sucrose (256.9 mg/15mM), Ferrous chloride (121.65 mg/15mM)and thiosulphate (186.1 mg/15mM).Nitrogen gas was bubbled into each bottle for anaerobic condition and sample VITRJS5 strain was inoculated in each bottle. These bottles were kept for overnight incubation in a shaker incubator. Narrowing down the number of donors based on the growth of sample. Different concentrations of selected donor are determined. Selected donors are added in different concentration of 5mM, 10mM, 15mM, 20mM and 30 mM. The steps for anaerobic culture are repeated. The absorbance is checked in UV-VIS spectr ophotometer at 595 nm. 2.4. Determination of nitrogen source for optimum growth: MS-1 Media was prepared without nitrogen source (MS-1 media without NH4H2PO4 ) and with the selected concentration of electron donor and acceptor. For this, activity of NH4H2PO4 was checked in different concentrations (5mM, 10mM, 15mM, 20mM and 30 mM) against different concentrations of NH4Cl(5mM, 10mM, 15mM, 20mM and 30 mM).The above described procedure of anaerobic growth was repeated. The absorbance was checked at a wavelength of 595 nm. 2. 5. Determination of reducing for optimum growth: MS-1 media with selected concentration of donor and acceptor was prepared. For this, activity of thiol glycolate was checked in different concentrations of 5mM, 10mM, 15mM, 20mM and 30 mM against cysteine-HCl 4% at concentrations of 50  µl , 100  µl, 150  µl, 200  µl and 500  µl. The procedure for anaerobic culture growth was repeated. The absorbance was checked at 595 nm. 2.6. Determination of iron source for optimum growth MS-1 media with selected concentration of donor and acceptor and without FeSO4 . 7H2O was prepared. For this, activity of ferrous sulphate was checked in different concentrations of 5mM, 10mM and 15mM against ferric citrate at concentrations of 0.5mM, 1.0mM and 1.5mM and against ferric quinate at concentrations of 0.5mM, 1.0mM and 1.5mM. The procedure for anaerobic culture growth was repeated. The absorbance was checked at 595 nm. 3. Result: 3.1 Electron acceptor and its concentration for optimum growth: On incubating the strain M.VITRJS5 in the MS1 media with the various electron acceptors, we obtained various results as seen in table1. From the table, we find that Nitrite, Nitrate and Selenite good electron acceptors for the strain. But we negate the role of Nitrite and Selenite as in nitrite, growth is observed after 2 weeks and selenite is considered toxic. Thus, Nitrate is considered as the most probable electron donor. Next, different concentrations of Nitrate are added to MS1 media to see which concentration of nitrate supports maximum growth (as seen in Table 2 and fig.1) Table 1. Tabular representation of growth with various electron acceptor. Table 2. Tabular representation of absorbance of different concentration of nitrate Fig.1 Graphical representation of the growth with different concentration of nitrate From the above table and fig.1, it is seen that 5mM of nitrate shows maximum growth on all three days. Thus, there is optimum growth seen in 5mM serum bottle. 3.2. Electron donor and its concentration for optimum growth On incubating the strain M.VITRJS5 in the MS1 media with the various electron  donors, we obtained various results as seen in table1.From the table 3, it is seen that growth of M.VITRJS5 is more pronounced with electron donors Ethanol, Glycerol, Pyruvate, Succinate, Lactate, Glucose and Sucrose along with Acetate which was already being used as an electron donor for other MTBs. Among these, Pyruvate, Sucrose and Acetate showed almost double the growth as seen in other electron donors. But we negate Sucrose as an electron donor as it is not economical. Later, different concentrations of Nitrate are added to MS1 media to see which concentration of nitrate supports maximum growth (as seen in Table 2 and fig.1) Table3. Representation of growth with various donors Table 4. Tabular Representation of absorbance of growth with different concentrations of pyruvate Fig 2. Graphical representation of absorbance of growth with different concentrations of pyruvate As seen in the above two graphs and tables, 30mM concentration of pyruvate shows highest growth in comparison with the other concentrations of acetate and pyruvate. 3. Nitrogen source and its concentration for optimum growth: The next step is incubation of M.VITRJS5 strain in MS-1 media(without NH4H2PO4) with Ammonium chloride and Ammonium hydrogen phosphate to determine the better nitrogen source. Fig 4. Graphical representation of absorbance of growth with different concentrations of NH4H2PO4 As seen in figure 4, 5 and tables 6,7, it is observed that the best growth is seen in bottles of Ammonium hydrogen phosphate(NH4H2PO4) rather than that of Ammonium chloride(NH4Cl). The most consistence growth is seen in 20 mM concentration of NH4H2PO4 as compared with other concentrations. 3.4. Carbon source: Since it has already been confirmed that pyruvate acts as the best electron donor, the same can act as a carbon source. 3.5. Phosphorus source: Ammonium hydrogen phosphate acts both as a nitrogen source as well as phosphorys source. 3.6. Sulphur source: Next the M.VITRJS5 strain was incubated in prepared MS-1 media with Sodium thioglycolate and cysteine HCl 4% to determine the better sulphur source. Fig 6. Graphical representation of absorbance of growth with different concentrations of Sodium thioglycolate As seen in fig 6,7 and table 8,9, 50 µl concentration of Cys –HCl shows highest growth as compared to the other concentrations. There was no growth in other concentrations due to the reducing properties of Cysteine HCl and Sodium thioglycolate. MTBs are microaerophilic and hence, would not grow in anaerobic condition. Sulphur is a reducing agent and hence, would remove oxygen species from the media. Therefore, there is less or minimal growth of the bacteria. 3.7 Iron source : Table 10. Tabular representation of absorbance of growth with different concentrations of Ferric citrate Fig 8. Graphical representation of absorbance of growth with different concentrations of Ferric citrate Table 11. Tabular representation of absorbance of growth with different concentrations of quinate Fig 9. Graphical representation of absorbance of growth with different concentrations of Ferric quinate 3.8 Comparison of new media with existing media: 4. Discussion: As already mentioned above, Magnetotactic bacteria (MTBs) are microaerophilic bacteria which have the ability to biomineralise membrane-encased, single-magnetic-domain mineral crystals (magnetosomes) and hence, cause the cell to orientate along the Earth’s geomagnetic field. These mostly occur in aquatic bodies ranging from saline to freshwater environment. Our basic objective in this study was to test the growth of the microaerophilic strain using different concentrations of electron donor, electron acceptor, nitrogen source, carbon source, phosphorus source, sulphur source, iron source, etc. From the study, the following observations have been noted down: The strain M.VITRJS5 was seen to grow well in 5mM concentration of Nitrate (electron acceptor), 30mM concentration of Pyruvate (electron donor and carbon source) and 20mM concentration of Ammonium hydrogen phosphate (nitrogen source and phosphorus source). Also, the growth of the strain was seen to be minimalized in case of Sodium thioglycolate and Cysteine HCl as these are strong reducing agents and thus, would reduce the amount of oxygen species in the media. As mentioned above, these bacteria are microaerophilic and hence, would not grow in sulphur sources (except in 15mM Sodium Thioglycolate and 50 µl Cysteine HCl). The comparison of the present media for Magnetospirillum growth media (MSGM) Schà ¼ler Magnetospirillum isolation media and the new prepared media was done. The new media contained sodium nitrate as acceptor, sodium pyruvate as electron donor and carbon source, ammonium hydrogen phosphate as nitrogen and phosphorus source, ferric citrate as iron source and cysteine HCl as sulphur source. Magnetotactic bacteria has the ability to produce magnetosomes which has stimulated and motivated a new field of research involving scientific and biomedical applications of MTB and diverse commercial which could be improved using magnetic nanocrystals. But the main drawbacks to the application of magnetosomes involves the fastidious nature of MTB related to the growth. Thus,it is difficult to culture them on a large scale, and it is important to understand genetic/environmental control for magnetosome synthesis.

What is a sample?

Whereas a population is the entire group of objects that a certain researcher is interested in; a sample is defined as the fixed number of objects you get from a certain population. For example, Amelia wants to know if red flowers attract bees more than yellow flowers. In order to prove this assumption, Amelia takes a sample of a red flower (e. g. rose) and a yellow flower (e. g. a sunflower). There are lots of flowers that are red and yellow in color. Amelia could not afford to obtain every red and yellow flower in order to prove her assumption.Thus, it is practical for her to take a â€Å"representative† from all the red flowers and a â€Å"representative† from all the yellow flowers. Taking â€Å"representatives† from the entire population, you could now call these â€Å"samples†. It is essential to remember that the fundamental assumption underlying most of the theory of sampling is random sampling. This consists of the selection of individuals from th e population in such a way that each individual of the population has an equal chance of being selected. The process of such selection is called random sampling.The aim of the theory of sampling is to get as much information as possible, ideally all the information about the population from which the sample has been drawn. From the parent population, in particular, we would like to estimate the parameters of the population or specify the limits or ranges within which the population parameters are expected to lie with a specified degree of confidence. At work, we use sampling to prove or test something. For example, you want to determine if the new time management scheme will be beneficial to cut the costs on your company.So, as a manager, you could take some employees to undergo this new time management scheme in order to see if the new process is suitable for both the company and the employees. 2. What are the differences between the binomial and normal distributions? What are the similarities between the binomial and normal distributions? The normal distribution is the most commonly encountered distribution range in science. Random variables in normal distribution should be capable of assuming any value on the real number line, though this requirement is often not applied.For example, height at a given age for a given gender in a given racial group is adequately described by a normal random variable even though heights must be positive. A continuous random variable X, taking all real values in the range. The graph of variables with normal distribution is a symmetrical, bell-shaped curve, centered at its expected mean value. Typically, a binomial random variable is the number of successions in a series of trials in binomial distributions.For example, the number of ‘heads' occurring when a coin is tossed 50 times; thus a discrete random variable X is said to follow a binomial distribution with parameters n and p. However, the probability trials must meet the following requirements: a. the total number of trials is fixed in advance; b. there are just two outcomes of each trial; success and failure; c. the outcomes of all the trials are statistically independent; d. all the trials have the same probability of success. The similarity of normal and binomial distributions rely on the use of random variables as part of the data and their values could be both positive and negative.3. What do confidence intervals represent? Give an example of the use of a confidence interval. Before a simple research question could be resolved like, for instance, â€Å"What is the mean number of flowers that one person can remember? † it is necessary to specify the population of people to which this question will be addressed. The researcher could be interested in, for example, children under the age of 12 and girls. For the present example, assume the researcher is interested in all girls aged 9. Once the population is specified, the next step is t o take a random sample from it.In this example, let's say that a sample of 10 girls is drawn and each student's memory tested. The way to estimate the mean of all girls would be to compute the mean of the 10 girls in the sample. Indeed, the sample mean is an unbiased estimate of ? , the population mean. However, it will certainly not be a perfect estimate. By chance it is bound to be at least either a little bit too high or a little bit too low. For the estimate of ? to be of value, one must have some idea of how precise it is. That is, how close to ? is the estimate likely to be?So we use the confidence intervals to determine how close would be the unbiased estimate we have in our sample to the values that is indicated in the population mean. If the number of flowers that the 10 girls remembered were: 4, 4, 5, 5, 5, 6, 6, 7, 8, 9 then the estimated value of ? would be 5. 9 and the 95% confidence interval would range from 4. 71 to 7. 09. The wider the interval, the more confident yo u are that it contains the parameter you are interested in. The 99% confidence interval is therefore wider than the 95% confidence interval and extends from 4. 19 to 7. 61.

Between Realism and Idealism

A great deal of conflict in the social life of individual people has been brought about by the existence of these concepts raising some questions of importance. Should an individual aim so high to an extent that is impossible to reach or aim average that is achievable?Should the individual assume on optimistic stand or a pessimistic one? Should the individual be good to others or fair to them? The existence of the various branches with regard to these categories proves that idealism or realism is never right with respect to all the branches.The issue of idealism and realism as a debate addresses the issues of whether an individual’s life as well as actions should be good as has been offered by the dogma of the Christians way of life. The issue of whether the individual should possess a caring sense as well as be fair with regard to the daily life is a determinant of whether the individual is living in a realistic or in an idealistic way of life.As for example, the issue of whe ther an individual an individual should drink or not depends on the stand the individual has taken with regard to the two extremes. It is evident that a person can drink and do no evil to the others while another person who does not drink at all may cause harm of significantly high magnitude to the life of others.It is a clear fact that each individual person is potentially capable for being good internally. In a realistic sense however some people are so static with regard to matters of change. As pertains the issue of whether the individual should drink or not, the goodness or the fairness of this will highly depend on the stand that the individual has taken.It is a possibility that one individual drinks and commits no harm to others while another person refraining from the activity may be of great trouble to other people. Furthermore the Christian Bible does not condemn drinking as an activity, but drunkardness as the effect of the activity (Tomuschat 83).As a clear guide as to t he best stand to take in life, a conservative approach to life is the most noble approach whereby the actions of the individual are determined by the need to be fair, obey the rule of justice as well as equality in a real life situation. It is good to make all the people get what is right for them, treating everybody in the same way and judging everybody the actions responsible for the person.The decision by the individual to arrange for a Sunday trip will also depend on the stand that the individual has taken since the trip may exhibit different motives. A trip that is meant for good as well as for the service of others is justified even at the context of the bible (Tomuschat 113).The contrasting belief is for people to live in a life of goodness, mercy as well as equality bearing in mind that every person bears intelligence which is a guide towards the actions taken, all of which are backed by some reason.The capacity for understanding is a clear lead to goodness. A fair treatment to all the people is important sign if the individual underwent a bad life in the childhood or is subjected to unconducive circumstances that fall beyond the control of the individual is a result of poverty wages, there occur a requirement for compensation so that everyone enjoys life in an equal capacity. The negligence of other people is responsible for the actions of others in the light of the considerations of the fallibility of people. Human beings are prone to making mistakes and this is so natural.A good person may be apprehended and aligned in the court of law for having committed a mistake. In the light of this argument, the possession of a Christian wife or otherwise similarly depends on the stand of the individual wife in as far as the two extremes of realism and idealism are concerned. The Christian wife is supposed to be a model for idealism, a stand which if defeated by realism, which bears potent influence to the behavior as well as the action of human, is equivalent with having a wife who is not a Christian (Tomuschat 213).ConclusionIn the consideration of the argument between justice and goodness, non is comparable to what God is in a position to handle which is usually in a perfect way and being neither rebel nor conservative and offering a question less situation as regards the correct balance in existence between the boundaries of justice on one side and grace on the other.In the case of the human being, who posses the characteristics of inferiority with respect to several aspects they can only grasp these concepts independently and one at every opportune time thus facilitating into parties of different orientations. It therefore important for an individual to lead a life of self awareness, that is instrumental towards the discovery of the stand of the individual with respect to idealism or realism.Work citedChristian, Tomuschat, Between Idealism & Realism. 2nd ed. USA: Oxford UniversityPress, 2004.